Indian Journal of Medical Microbiology

Fluorescent in situ hybridization (FISH) was carried out using two different oligonucleotide probes speciÞ c for Pseudomonas spp. and Acinetobacter spp. These probes were tested against different organisms and were found to be highly speciÞ c. Sensitivity testing showed that the probes were able to detect as low as 103 CFU/mL. In addition, FISH was carried out directly on positive blood culture samples and the detection of microorganisms took less than 2 h. We believe that FISH is a rapid method that can be used as a routine laboratory diagnostic technique for the detection of Acinetobacter spp. and Pseudomonas spp. in clinical samples.